Quantitative Chemiluminiscent Detection of Plant Peroxidases Using a Commercial Kit Originally Designed for Blotting Assays
Abstrakt
The paper describes an improvement of chemiluminescent analysis of plant peroxidases by the use of a commercial kit originally designed for qualitative Western blotting assays. The chemiluminescent method using the reagents of the Amersham ECL kit offers a good alternative to the well known and widely exploited colorimetric or chemiluminescent methods using luminol. The proposed procedure significantly improves the chemiluminescent enzyme assay with labour-consuming separate mixing of all components by application of standard and ready-to-use solutions.Stahování
Publikováno
15.03.2001
Jak citovat
Mackova, M., Ferri, E., Demnerova, K., & Macek, T. (2001). Quantitative Chemiluminiscent Detection of Plant Peroxidases Using a Commercial Kit Originally Designed for Blotting Assays. Chemické Listy, 95(2). Získáno z http://www-.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/2449
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